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Royal Society of Chemistry, Molecular BioSystems, 1(13), p. 92-105

DOI: 10.1039/c6mb00701e

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Proteomics profiling of interactome dynamics by colocalisation analysis (COLA).

Journal article published in 2016 by Faraz K. Mardakheh, Heba Z. Sailem, Sandra Kümper, Christopher J. Tape ORCID, Ryan R. McCully, Angela Paul, Sara Anjomani-Virmouni, Claus Jørgensen ORCID, George Poulogiannis, Christopher J. Marshall, Chris Bakal ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Localisation and protein function are intimately linked in eukaryotes, as proteins are localised to specific compartments where they come into proximity of other functionally relevant proteins. Significant co-localisation of two proteins can therefore be indicative of their functional association. We here present COLA, a proteomics based strategy coupled with a bioinformatics framework to detect protein-protein co-localisations on a global scale. COLA reveals functional interactions by matching proteins with significant similarity in their subcellular localisation signatures. The rapid nature of COLA allows mapping of interactome dynamics across different conditions or treatments with high precision.