A flow injection with chemiluminescence detection (FI-CL) method was used to determine the concentration of dissolved iron in seawater samples collected in the South Atlantic during the GEOTRACES GA10 cruise that took place from 24th December 2011 – 27th January 2012 on board the R.R.S. James Cook (cruise JC068). Open ocean (shallow and deep) and coastal (shallow and deep) samples were collected and five sub-samples from each collection were filtered through a cartridge filter. For the deep open ocean sample, separate sub-samples were also filtered through a membrane disc filter. In addition, deep open ocean sub-samples were also taken from five separate sampling bottles. Each sub-sample (29 in total) was analysed six times (giving 174 discrete measurements in total) and the within sub-sample precision was in the range 1.4 – 12.2%. There was no statistically significant difference for the deep, open ocean sample between the mean results obtained with the two different filter types or the single sample bottle versus separate sample bottle sub-samples. The between sub-sample measurement variability included uncertainty contributions from sample collection and filtration. Further statistical evaluation using ANOVA showed that the relative combined standard uncertainty accounting only for this component and the short-term analytical repeatability (i.e. replicate FI-CL measurements on a sub-sample) ranged from 2.3% - 3.8%. ; JRC.F.6-Reference Materials