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Cambridge University Press, Lichenologist, 2(32), p. 189-196

DOI: 10.1006/lich.1999.0254

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Alternative Methods of Extracting and Amplifying Dna From Lichens

Journal article published in 2000 by Maria P. Martín ORCID, Katarina Winka
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

Full text: Unavailable

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Abstract

AbstractWe have investigated whether DNA extraction protocols designed specifically for fungi and/or lichens perform better on lichens than do corresponding protocols designed for plants and insects. Two different PCR-amplification protocols were used to evaluate the quality of the DNA extracted with each method. The DNA extractions with highest quality were obtained with the protocols designed for insects and plants, and the most successful amplifications were obtained with Ready-To-Go PCR Beads. This indicates that fungal or lichen specific protocols might not be necessary for successful extraction of high quality DNA from lichens.