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Royal Society of Chemistry, Chemical Communications, 1(47), p. 400-402

DOI: 10.1039/c0cc01707h

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Measuring the Stoichiometry of Functional PspA Complexes in Living Bacterial Cells by Single Molecule Photobleaching†

This paper is available in a repository.
This paper is available in a repository.

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Abstract

We report a general method based on wide-field fluorescence imaging of single molecule photobleaching and the Chung-Kennedy algorithm to measure the stoichiometry of functional protein complexes in living bacterial cells.