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Royal Society of Chemistry, Lab on a Chip, 3(12), p. 659-661, 2012

DOI: 10.1039/c2lc20515g

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Multi-gradient hydrogels produced layer by layer with capillary flow and crosslinking in open microchannels

Journal article published in 2011 by Francesco Piraino, Gulden Camci-Unal, Matthew J. Hancock, Marco Rasponi ORCID, Ali Khademhosseini
This paper is available in a repository.
This paper is available in a repository.

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Abstract

This technical note describes a new bench-top method for producing anisotropic hydrogels composed of gradient layers of soluble factors, particles, polymer concentrations or material properties. Each gradient layer was produced by a previous gradient method in which a droplet of one precursor solution was added to a thin layer of a second solution. The ensuing rapid capillary flow along the open channel generated a gradient precursor solution, which was then crosslinked to form a gradient gel. Repeating these steps allowed a layered gel to be iteratively constructed with as many gradient layers as desired. This technique renders the synthesis of multi-layered gradient gels accessible to virtually any researcher and should help simplify the production of more biologically relevant cellular microenvironments.