Published in
Royal Society of Chemistry, Organic and Biomolecular Chemistry, 45(10), p. 9021
DOI: 10.1039/c2ob25751c
Links
Tools
Rational substrate and enzyme engineering of transketolase for aromatics
Journal article published in 2012 by
Panwajee Payongsri,
David Steadman 
,
John Strafford,
Andrew MacMurray,
Hc Hailes,
Pa Dalby
,
John Strafford,
Andrew MacMurray,
Hc Hailes,
Pa Dalby
This paper is available in a repository.
Full text: Download
Published version:
archiving forbidden
Data provided by 
Abstract
The uses of 3-formylbenzoic acid and 4-formylbenzoic acid as molecular probes along with previous and new transketolase mutants revealed the factors governing the rate of reaction between transketolase and aromatic aldehydes. The novel α,α-dihydroxyketones were produced at 15 to 30-fold higher yields and up to 250-fold higher specific activities with D469T TK when compared to those obtained for benzaldehyde.