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American Society for Microbiology, Molecular and Cellular Biology, 17(21), p. 6006-6016, 2001

DOI: 10.1128/mcb.21.17.6006-6016.2001

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Mre11 Complex and DNA Replication: Linkage to E2F and Sites of DNA Synthesis†

Journal article published in 2001 by Richard S. Maser, Olga K. Mirzoeva, Julie Wells, Heidi Olivares, Bret R. Williams ORCID, Robert A. Zinkel, Peggy J. Farnham ORCID, John H. J. Petrini
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

We show that the Mre11 complex associates with E2F family members via the Nbs1 N terminus. This association and Nbs1 phosphorylation are correlated with S-phase checkpoint proficiency, whereas neither is sufficient individually for checkpoint activation. The Nbs1 E2F interaction occurred near the Epstein-Barr virus origin of replication as well as near a chromosomal replication origin in the c-myc promoter region and was restricted to S-phase cells. The Mre11 complex colocalized with PCNA at replication forks throughout S phase, both prior to and coincident with the appearance of nascent DNA. These data suggest that the Mre11 complex suppresses genomic instability through its influence on both the regulation and progression of DNA replication.