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Elsevier, Journal of Biological Chemistry, 36(285), p. 28220-28228, 2010

DOI: 10.1074/jbc.m110.118554

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Clostridiolysin S, a Post-translationally Modified Biotoxin fromClostridium botulinum

Journal article published in 2010 by David J. Gonzalez, Shaun W. Lee, Mary E. Hensler, Andrew L. Markley, Samira Dahesh, Douglas A. Mitchell ORCID, Nuno Bandeira, Victor Nizet, Jack E. Dixon, Pieter C. Dorrestein
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Through elaboration of its botulinum toxins, Clostridium botulinum produces clinical syndromes of infant botulism, wound botulism, and other invasive infections. Using comparative genomic analysis, an orphan nine-gene cluster was identified in C. botulinum and the related foodborne pathogen Clostridium sporogenes that resembled the biosynthetic machinery for streptolysin S, a key virulence factor from group A Streptococcus responsible for its hallmark beta-hemolytic phenotype. Genetic complementation, in vitro reconstitution, mass spectral analysis, and plasmid intergrational mutagenesis demonstrate that the streptolysin S-like gene cluster from Clostridium sp. is responsible for the biogenesis of a novel post-translationally modified hemolytic toxin, clostridiolysin S.