Royal Society of Chemistry, Organic and Biomolecular Chemistry, 10(14), p. 2938-2943, 2016
DOI: 10.1039/c5ob02358k
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G-quadruplex structures formed by oligodeoxyribonucleotides TGGUNH2GGT (AM, UNH2 = 5-amino-2'-deoxyuridine), TGGUBrGGT (BR, UBr = 5-bromo-2'-deoxyuridine) and TGGTGGT (TH) have been investigated through circular dichroism, nuclear magnetic resonance, gel electrophoresis and molecular modeling techniques. Collected data indicate that all 7-mer oligonucleotides form tetramolecular parallel G-quadruplex structures with all residues adopting anti glycosidic bonds. In the case of AM, data suggest the occurrence of a novel UNH2-tetrad characterized by eight hydrogen bonds and stabilizing the G-quadruplex structure more efficiently than UBr- and T-tetrads.