Royal Society of Chemistry, Chemical Communications, 55(49), p. 6164
DOI: 10.1039/c3cc42790k
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A direct ELISA was established for the fast detection of semicarbazide (SEM) using a novel biotin derivative. Without a tedious extraction procedure, as low as 0.07 μg L(-1) of SEM could be detected reproducibly. This assay has better recovery and accuracy than competitive ELISA.