Published in
Royal Society of Chemistry, Chemical Communications, 25(51), p. 5250-5252
DOI: 10.1039/c4cc07974d
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Collagen labelling with an azide-proline chemical reporter in live cells
Journal article published in 2014 by
Beatrice Amgarten,
Rakesh Rajan,
Nuria Martínez-Sáez,
Bruno L. Oliveira 
,
Inês S. Albuquerque,
Roger A. Brooks,
David G. Reid,
Melinda J. Duer,
Gonçalo J. L. Bernardes
,
Inês S. Albuquerque,
Roger A. Brooks,
David G. Reid,
Melinda J. Duer,
Gonçalo J. L. Bernardes
This paper is made freely available by the publisher.
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Abstract
We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide-alkyne cycloaddition reaction.