Published in
Royal Society of Chemistry, Lab on a Chip, 1(13), p. 68-72
DOI: 10.1039/c2lc40948h
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Luminometric sub-nanoliter droplet-to-droplet array (LUMDA) and its application to drug screening by phase I metabolism enzymes
Journal article published in 2012 by
Giuseppe Arrabito 
,
Clelia Galati,
Sabrina Castellano,
Bruno Pignataro
,
Clelia Galati,
Sabrina Castellano,
Bruno Pignataro
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Abstract
Here we show the fabrication of the Luminometric Sub-nanoliter Droplet-to-droplet Array (LUMDA chip) by inkjet printing. The chip is easy to be implemented and allows for a multiplexed multi-step biochemical assay in sub-nanoliter liquid spots. This concept is here applied to the integral membrane enzyme CYP3A4, i.e. the most relevant enzymatic target for phase I drug metabolism, and to some structurally-related inhibitors.