Published in
Royal Society of Chemistry, Chemical Communications, 48(48), p. 6037
DOI: 10.1039/c2cc31107k
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Amphipol mediated surface immobilization of FhuA: A platform for label-free detection of the bacteriophage protein pb5
Journal article published in 2012 by
Hajra Basit,
K. Shivaji Sharma,
Angéline Van der Heyden,
Chantal Gondran,
Cécile Breyton,
Pascal Dumy 
,
Françoise M. Winnik,
Pierre Labbé
,
Françoise M. Winnik,
Pierre Labbé
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Abstract
Biotinylated amphipol was used to entrap FhuA (an E. coli outer membrane protein) and immobilize the FhuA-amphipol complex on streptavidin surfaces. Using this assembly, we have successfully devised surface-based assays for studying the recognition of FhuA by pb5 (a bacteriophage T5 protein) and determination of the affinity constant.