Royal Society of Chemistry, Chemical Communications, 11(48), p. 1701-1703, 2012
DOI: 10.1039/c2cc16537f
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A new surface-based MALDI-Tof-MS glycosyl hydrolase assay has been developed in which lipid-tagged oligosaccharides, representing defined fragments of major plant cell wall polysaccharides, are immobilized via hydrophobic interactions on an alkylthiol functionalised gold sample plate and employed in the functional screening of several purified enzymes, environmental samples and saliva.