Single crystal of L-Prolinium Tartrate (LPT), which is an organic non linear optical material was successfully synthesized and grown by slow evaporation solution growth technique (SEST). The crystal structure and its lattice parameters of the grown crystal was confirmed by powder X-ray diffraction technique and found that it belongs to monoclinic crystal system having β= 100.380 with noncentrosymmetric space group. The presence of strain in the grown ingot was also calculated from the powder X-ray diffraction measurements. The crystalline perfection was examined by high resolution X-ray diffractometry, which reveals that the crystal contains structural the grain boundaries. The optical behavior of the grown specimen was analyzed by photoluminescence and calculated its time resolved PL decay. Grown crystal has adopted the step wise growth pattern having parallel striations, which is confirmed from etching technique. Its ferroelectric and piezoelectric properties have been assessed. Its third order non linearity was assessed by using open aperture Z-Scan technique. The thermal parameters of LPT single crystal have been calculated by using photopyroelectric technique. The mechanical strength of single crystal at micro level is observed by nanoindentation using Oliver- Pharr method.