Published in
Royal Society of Chemistry, Analyst, 22(136), p. 4670, 2011
DOI: 10.1039/c1an15684e
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High throughput plasma N-glycome profiling using multiplexed labelling and UPLC with fluorescence detection
Journal article published in 2011 by
Ana Knežević,
Jonathan Bones,
Stjepan Krešimir Kračun,
Olga Gornik,
Pauline M. Rudd,
Gordan Lauc 
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Abstract
A rapid glycomic profiling method is described wherein N-glycans from plasma samples individually labelled with aniline, 2-aminobenzamide and 2-aminoacridone are mixed, co-injected and separated in the same HILIC-fluorescence run. Transfer of the multiplexed method to UPLC-fluorescence permits an increase in sample throughput from 24 to 864 plasma samples per day.