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Royal Society of Chemistry, Molecular BioSystems, 9(5), p. 931

DOI: 10.1039/b904228h

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Improving orthogonal tRNA-synthetase recognition for efficient unnatural amino acid incorporation and application in mammalian cells

Journal article published in 2009 by Jeffrey K. Takimoto, Katrina L. Adams, Zheng Xiang, Lei Wang ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Optimizing the anticodon recognition between orthogonal tRNA and synthetase significantly increased the incorporation efficiencies of various unnatural amino acids in mammalian cells, and the enhanced incorporation enabled efficient photocrosslinking of interacting proteins in mammalian cells.