Published in
Royal Society of Chemistry, Chemical Communications, 25(51), p. 5343-5346, 2015
DOI: 10.1039/c4cc09287b
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The Best of Both Worlds: Active Enzymes by Grafting-To followed by Grafting-From a Protein
Journal article published in 2014 by
Rebecca Falatach,
M. Sameer Al Abdul Wahid,
Cameron McGlone,
M. Sameer Al-Abdul-Wahid,
Saadyah Averick,
Richard C. Page 
,
Jason A. Berberich,
Dominik Konkolewicz
,
Jason A. Berberich,
Dominik Konkolewicz
This paper is available in a repository.
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Abstract
Hydrophilic polymers were attached to lysozyme by a combination of grafting-to and grafting-from approaches using RAFT polymerization. A hydrophilic oligomer was synthesized, and attached to the protein. The protein-oligomer hybrid contained the RAFT end group, enabling chain extension in solution. Lysozyme maintained activity throughout this process.