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Royal Society of Chemistry, Green Chemistry, 10(16), p. 4520-4523

DOI: 10.1039/c4gc01329h

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Structural–functional evaluation of ionic liquid libraries for the design of co-solvents in lipase-catalysed reactions

Journal article published in 2014 by João V. Rodrigues ORCID, Diana Ruivo, Ana Rodríguez, Francisco J. Deive, José M. S. S. Esperança ORCID, Isabel M. Marrucho, Cláudio M. Gomes ORCID, Luís Paulo N. Rebelo ORCID
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This paper is available in a repository.

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Abstract

Using ionic liquids as co-solvents may improve reaction media in enzyme-based biotechnological processes. To establish new conditions, large libraries need to be screened for bio-compatibility and protein stabilisation. Using a lipase model, we herein describe a combination of methods leading to an expedited evaluation of 61 different solvent compositions.