Thin and compliant conductive materials and electronic devices that are able to stand as free-standing membranes or to conform to surfaces are relevant for the development of human-device interfaces and unperceivable skin-contact personal health monitoring systems. In this work, a roll-to-roll (R2R) process for the preparation of conductive polymer nanosheets on large areas has been developed in view to move such technology towards real-world applications. R2R conductive nanosheets are obtained as free-standing structures through release from a temporary substrate and then transferred in conformal contact to any target surface with arbitrary shape, curvature and surface topography (including biological tissue such as skin). A specific high-conductivity formulation of PEDOT:PSS has been optimized for skin-contact applications, by making use of butylene glycol (BG) as dopant: a dermatologically approved ingredient. The R2R nanosheets were tested as unperceivable surface electromyography electrodes able to record muscle electric activity. The present R2R process has advantageous properties such as continuous, high throughput printing on large area rolls, cost-effectiveness, speed of execution and use of industry-ready/mass-scale manufacturing technology.