Published in
Royal Society of Chemistry, Soft Matter, 39(11), p. 7707-7711
DOI: 10.1039/c5sm01490e
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Grafted biomembranes containing membrane proteins – the case of the leucine transporter
Journal article published in 2015 by
Vivien Jagalski,
Robert D. Barker,
Mikkel B. Thygesen,
Kamil Gotfryd,
Mie B. Krüger,
Lei Shi,
Selma Maric 
,
Nicolas Bovet,
Martine Moulin,
Michael Haertlein,
Thomas Günther Pomorski,
Claus J. Loland,
Marité Cárdenas
,
Nicolas Bovet,
Martine Moulin,
Michael Haertlein,
Thomas Günther Pomorski,
Claus J. Loland,
Marité Cárdenas
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Abstract
Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergent and perform a detergent-lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron reflection using the predicted model from molecular dynamic simulations.