A pot experiment was conducted to evaluate the role of glycinebetaine (GB) in chromium (Cr) tolerance in mung bean (Vigna radiata L.) grown in Cr-stressed soil. Three concentrations of Cr (0, 250 and 500 µM) were tested with three (0, 50 and 100 mM) concentrations of foliar-applied GB. Cr alone led to a significant decrease in plant growth, biomass, and concentrations of chlorophyll a, b and carotenoids. Cr concentration and electrolyte leakage significantly increased in plants with increasing Cr levels in the soil. Lower Cr stress enhanced the activities of superoxide dismutase (SOD), peroxidases (POD) and catalase (CAT), while higher Cr concentrations decreased the activities of these enzymes. Foliar application of GB successfully alleviated toxic effects of Cr on mung bean and increased plant growth, biomass and chlorophyll contents under Cr stress. GB application reduced Cr accumulation and electrolyte leakage in plants and enhanced the activities of antioxidant enzymes in both shoots and roots as compared with Cr treatments alone. These findings suggest that foliar-applied GB alleviated Cr-induced oxidative stress in mung bean by reducing Cr uptake. The protective effect of GB against Cr stress varies with the concentrations of GB and Cr stress applied. Thus, further studies are still needed to specify the concentrations of GB required for detoxification of specific Cr concentrations under various climatic conditions.