Royal Society of Chemistry, Lab on a Chip, 24(13), p. 4740
DOI: 10.1039/c3lc50945a
Full text: Download
Here, we present a platform to detect cytokine (IL-2, IFN-gamma, TNF-alpha) secretion of single, activated T-cells in droplets over time. We use a novel droplet-based microfluidic approach to encapsulate cells in monodisperse agarose droplets together with functionalized cytokine-capture beads for subsequent binding and detection of secreted cytokines from single cells. This method allows high-throughput detection of cellular heterogeneity and maps subsets within cell populations with specific functions.