Published in
Royal Society of Chemistry, Lab on a Chip, 16(13), p. 3183, 2013
DOI: 10.1039/c3lc50276g
Links
- ORCID
- Royal Society of Chemistry
- [www.ncbi.nlm.nih.gov] | PDF
- [europepmc.org] | PDF
- [www.researchgate.net] | PDF
- [www.ncbi.nlm.nih.gov] | PDF
Tools
A Microfluidic Approach for Protein Structure Determination at Room Temperature via on-chip Anomalous Diffraction
Journal article published in 2013 by
Sarah L. Perry,
Sudipto Guha,
Ashtamurthy S. Pawate,
Amrit Bhaskarla,
Vinayak Agarwal,
Satish K. Nair,
Paul J. A. Kenis 
This paper is available in a repository.
Full text: Download
Published version:
archiving forbidden
Data provided by 
Abstract
We report a microfluidic approach for de novo protein structure determination via crystallization screening and optimization, as well as on-chip X-ray diffraction data collection. The structure of phosphonoacetate hydrolase (PhnA) has been solved to 2.11 Å via on-chip collection of anomalous data that has an order of magnitude lower mosaicity than what is typical for traditional structure determination methods.