Scopatizzo, belonging to the Cucumis melo L., is a local variety of Apulia (Southern Italy), which is consumed as unripe melon as an alternative of cucumber due to its better-quality profile and for the absence of cucurbitacins. The latter are tetracyclic triterpenes synthesized by some Cucurbitaceae species, known to confer an unpleasant taste to fruits and cause health problems. Following the discovery of Scopatizzo fruits with bitter taste, cucurbitacins were searched for in their ethanolic extract. Flow injection analysis with detection performed by atmospheric pressure chemical ionization-high resolution mass spectrometry provided evidence for the presence of at least four cucurbitacins, which were absent in typical, sweet-tasting fruits. Further insight into this discovery will be required in the near future to assess if the detection of cucurbitacins may mark the appearance of genotypes whose fruits have features not compatible with commercialization.