Published in
Royal Society of Chemistry, Molecular BioSystems, 2(11), p. 379-383
DOI: 10.1039/c4mb00452c
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NMR analysis of budding yeast metabolomics: a rapid method for sample preparation
Journal article published in 2015 by
Cristina Airoldi 
,
Farida Tripodi,
Cinzia Guzzi,
Raffaele Nicastro,
Paola Coccetti
,
Farida Tripodi,
Cinzia Guzzi,
Raffaele Nicastro,
Paola Coccetti
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Abstract
Here we propose the optimization of a rapid and reproducible protocol for intracellular metabolite extraction from yeast cells and their metabolic profiling by 1H-NMR spectroscopy. The protocol reliability has been validated through the comparison between the metabolome of cells in different phases of growth or with different genetic backgrounds.