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Royal Society of Chemistry, Chemical Communications, 63(58), p. 8790-8793, 2022
DOI: 10.1039/d2cc02360a
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Pulse dipolar EPR for determining nanomolar binding affinities
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Abstract
CuII-nitroxide RIDME measurements at 100 nM protein concentration allow reliable extraction of dissociation constants and distances, while measurements at 50 nM protein concentration allow reliable extraction of dissociation constants only.