Abstract Knowledge of the stratigraphic development of pre-Carboniferous strata in the subsurface of the Netherlands is very limited, leaving the lithostratigraphic nomenclature for this time interval informal. In two wells from the southwestern Netherlands, Silurian strata have repeatedly been reported, suggesting that these are the oldest ever recovered in the Netherlands. The hypothesised presence of Silurian-aged strata has not been tested by biostratigraphic analysis. A similar lack of biostratigraphic control applies to the overlying Devonian succession. We present the results of a palynological study of core material from wells KTG-01 and S05-01. Relatively low-diversity and poorly preserved miospore associations were recorded. These, nonetheless, provide new insights into the regional stratigraphic development of the pre-Carboniferous of the SW Netherlands. The lower two cores from well KTG-01 are of a late Silurian (Ludlow–Pridoli Epoch) to earliest Devonian (Lochkovian) age, confirming that these are the oldest sedimentary strata ever recovered in the Netherlands. The results from the upper cored section from the pre-Carboniferous succession in well KTG-01 and the cored sections from the pre-Carboniferous succession in well S05-01 are more ambiguous. This inferred Devonian succession is, in the current informal lithostratigraphy of the Netherlands, assigned to the Banjaard group and its subordinate Bollen Claystone formation, of presumed Frasnian (i.e. early Late Devonian) age. Age-indicative Middle to Late Devonian palynomorphs were, however, not recorded, and the overall character of the poorly preserved palynological associations in wells KTG-01 and S05-01 may also suggest an Early Devonian age. In terms of lithofacies, however, the cores in well S05-01 can be correlated to the upper Frasnian – lower Famennian Falisolle Formation in the Campine Basin in Belgium. Hence, it remains plausible that an unconformity separates Silurian to Lower Devonian strata from Upper Devonian (Frasnian–Famennian) strata in the SW Netherlands. In general, the abundance of miospore associations points to the presence of a vegetated hinterland and a relatively proximal yet relatively deep marine setting during late Silurian and Early Devonian times. This differs markedly from the open marine depositional settings reported from the Brabant Massif area to the south in present-day Belgium, suggesting a sediment source to the north. The episodic presence of reworked (marine) acritarchs of Ordovician age suggests the influx of sedimentary material from uplifted elements on the present-day Brabant Massif to the south, possibly in relation to the activation of a Brabant Arch system.