Published in
Royal Society of Chemistry, Chemical Communications, 21(57), p. 2681-2684, 2021
DOI: 10.1039/d0cc08226k
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Understanding substrate binding and the role of gatekeeping residues in PigC access tunnels
Journal article published in 2021 by
Stefanie Brands 
,
Jarno G. Sikkens,
Mehdi D. Davari ,
Hannah U. C. Brass ,
Andreas Sebastian Klein ,
Jörg Pietruszka ,
Anna Joelle Ruff ,
Ulrich Schwaneberg
,
Jarno G. Sikkens,
Mehdi D. Davari ,
Hannah U. C. Brass ,
Andreas Sebastian Klein ,
Jörg Pietruszka ,
Anna Joelle Ruff ,
Ulrich Schwaneberg
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Abstract
Prodigiosin ligase PigC has been engineered by semi-rational design to accept short chain-pyrroles, providing molecular understanding of access tunnels and the substrate-binding pocket.