SummaryStudies have shown that daily exposure to different products, whether chemical or natural, can cause irreversible damage to women’s reproductive health. Therefore it is necessary to use tests that evaluate the safety and efficacy of these products. Most reproductive toxicology tests are performedin vivo. However, in recent years, various cell culture methods, including embryonic stem cells and tissues have been developed with the aim of reducing the use of animals in toxicological tests. This is a major advance in the area of toxicology, as these systems have the potential to become a widely used tool compared within vivotests routinely used in reproductive biology and toxicology. The present review describes and highlights data onin vitroculture processes used to evaluate reproductive toxicity as an alternative to traditional methods usingin vivotests.