Published in
Royal Society of Chemistry, Analyst, 24(138), p. 7294
DOI: 10.1039/c3an01540h
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Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers
Journal article published in 2013 by
Mark S. Friddin 
,
Friddin Ms,
Natalie P. Smithers,
Smithers Np,
Maïwenn Beaugrand,
Isabelle Marcotte,
Philip T. F. Williamson,
Williamson Pt,
Maurits R. R. de Planque,
Hywel Morgan ,
de Planque Mr
,
Friddin Ms,
Natalie P. Smithers,
Smithers Np,
Maïwenn Beaugrand,
Isabelle Marcotte,
Philip T. F. Williamson,
Williamson Pt,
Maurits R. R. de Planque,
Hywel Morgan ,
de Planque Mr
This paper is available in a repository.
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Abstract
Single-channel electrophysiology with lipid bilayer systems requires ion channel expression, purification from cell culture, and reconstitution in proteoliposomes for delivery to a planar bilayer. Here we demonstrate that single-channel current measurements of the potassium channels KcsA and hERGS5-S6 can be obtained by direct insertion in interdroplet lipid bilayers from microliters of a cell-free expression medium.