Published in
Royal Society of Chemistry, Organic and Biomolecular Chemistry, 20(17), p. 4964-4969, 2019
DOI: 10.1039/c9ob00508k
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TuPPL: Tub-tag mediated C-terminal protein–protein-ligation using complementary click-chemistry handles
Journal article published in 2019 by
Andreas Stengl 
,
Marcus Gerlach,
Marc-André Kasper,
Christian P. R. Hackenberger ,
Heinrich Leonhardt,
Dominik Schumacher ,
Jonas Helma
,
Marcus Gerlach,
Marc-André Kasper,
Christian P. R. Hackenberger ,
Heinrich Leonhardt,
Dominik Schumacher ,
Jonas Helma
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Abstract
We introduce a chemoenzymatic strategy for straightforward in vitro generation of C-terminally linked fusion proteins.