Asymmetric bifunctional protein nanoparticles through redesign of self-assembly
Journal article published in 2019 by
Santiago Sosa,
Andrés H. Rossi,
Alan M. Szalai,
Sebastián Klinke 
,
Jimena Rinaldi,
Ana Farias,
Paula M. Berguer,
Alejandro D. Nadra ,
Fernando D. Stefani ,
Fernando A. Goldbaum,
Hernán R. Bonomi
,
Jimena Rinaldi,
Ana Farias,
Paula M. Berguer,
Alejandro D. Nadra ,
Fernando D. Stefani ,
Fernando A. Goldbaum,
Hernán R. Bonomi
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Abstract
In this work we rationally redesign and engineer a decameric protein scaffold to generate an asymmetric Janus-like protein nanoparticle. As a proof of concept this novel scaffold is functionalized (i) to fluorescently label eukaryotic cells and (ii) to generate a multichromophoric FRET nanoparticle.