Published in
Royal Society of Chemistry, Lab on a Chip, 18(18), p. 2776-2786
DOI: 10.1039/c8lc00507a
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Microfluidic filter device with nylon mesh membranes efficiently dissociates cell aggregates and digested tissue into single cells
Journal article published in 2018 by
Xiaolong Qiu,
Jeremy A. Lombardo,
Trisha M. Westerhof 
,
Marissa Pennell,
Anita Ng,
Hamad Alshetaiwi,
Brian M. Luna ,
Edward L. Nelson,
Kai Kessenbrock,
Elliot E. Hui ,
Jered B. Haun
,
Marissa Pennell,
Anita Ng,
Hamad Alshetaiwi,
Brian M. Luna ,
Edward L. Nelson,
Kai Kessenbrock,
Elliot E. Hui ,
Jered B. Haun
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Abstract
Tissues are increasingly being analyzed at this single cell level. We present a simple and inexpensive microfluidic filter device that can rapidly and effectively improve the quality of single cell suspensions obtained from digested tissue samples.