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Springer Nature [academic journals on nature.com], Cell Death and Disease, 10(8), p. e3107-e3107, 2017

DOI: 10.1038/cddis.2017.497

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Gadd45a opens up the promoter regions of miR-295 facilitating pluripotency induction

Journal article published in 2017 by Linpeng Li, Keshi Chen ORCID, Yi Wu ORCID, Qi Long ORCID, Danyun Zhao, Bochao Ma, Duanqing Pei, Xingguo Liu
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

AbstractMicroRNAs (miRNAs) play crucial roles in the establishment of pluripotent state by controlling pluripotent network. However, the molecular mechanisms controlling miRNAs during somatic cell reprogramming remain obscure. In this study, we show Gadd45a (growth arrest and DNA-damage-inducible protein 45a) enhances reprogramming by activating miR-295. Furthermore, we show that Gadd45a binds the promoter regions of miR-295. Nuclease accessibility assay indicates that Gadd45a opens the promoter regions of miR-295. Levels of H3K9Ac and H3K27Ac on the promoter regions of miR-295 were also increased. In conclusion, our results indicate that Gadd45a relaxes the promoter regions of miR-295 and promotes the expression of miR-295 during reprogramming, implying a concise mechanism of Gadd45a and miR-290 cluster cooperation in cell-fate determination.