In order to study the flexural behavior of steel fiber reinforced concrete (SFRC) shield tunnel segment, two full scale shield tunnel segments and twelve reduced scale shield tunnel segments were made and tested. Based on the experiment study, the effectiveness of reduced scale segment was analyzed. Meanwhile, based on the fail mode, load-deflection curves and crack width, the part substitution of steel reinforcement by steel fibers was studied. Test results showed that the reduced scale model can reflect the flexural behavior of full scales tunnel segment and 45kg/m³ steel fibers of 3D type can replace 17% longitudinal reinforcement and 70% stirrups.