Dissemin is shutting down on January 1st, 2025

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American Society for Microbiology, Journal of Clinical Microbiology, 3(52), p. 957-959, 2014

DOI: 10.1128/jcm.03062-13

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Characterization of a Novel Melt Curve by Use of the Roche LightCycler HSV 1/2 Analyte-Specific Reagent Real-Time PCR Assay: Frequencies of This Novel (Low) Melt Curve and Commonly Encountered (Intermediate) Melt Curves

Journal article published in 2013 by Bobby L. Boyanton, Amro Almradi, Mark J. Espy, Anne E. Prada, John P. Gibson, Bobbi S. Pritt ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

ABSTRACT We characterize a novel probe binding-site polymorphism detectable solely by melt curve analysis using the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay. The frequencies of this novel (47°C) and previously described intermediate (60 to 62°C) melt curves were 0.016% and 4.9%, respectively.