Published in

Wiley, British Journal of Haematology, 5(142), p. 802-807, 2008

DOI: 10.1111/j.1365-2141.2008.07261.x

Links

Tools

Export citation

Search in Google Scholar

An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase

Journal article published in 2008 by Alexander Kohlmann, Thomas J. Kipps, Kipps Tj, Rassenti Lz, Laura Z. Rassenti, James R. Downing, Shurtleff Sa, Sheila A. Shurtleff, Mills Ki, Ken I. Mills, Amanda F. Gilkes, Gilkes Af, Wolf-Karsten Hofmann, Giuseppe Basso, Hofmann Wk and other authors.
This paper is available in a repository.
This paper is available in a repository.

Full text: Download

Green circle
Preprint: archiving allowed
Upload
Orange circle
Postprint: archiving restricted
Upload
Red circle
Published version: archiving forbidden
Policy details
Data provided by SHERPA/RoMEO

Abstract

Gene expression profiling has the potential to enhance current methods for the diagnosis of haematological malignancies. Here, we present data on 204 analyses from an international standardization programme that was conducted in 11 laboratories as a prephase to the Microarray Innovations in LEukemia (MILE) study. Each laboratory prepared two cell line samples, together with three replicate leukaemia patient lysates in two distinct stages: (i) a 5-d course of protocol training, and (ii) independent proficiency testing. Unsupervised, supervised, and r(2) correlation analyses demonstrated that microarray analysis can be performed with remarkably high intra-laboratory reproducibility and with comparable quality and reliability.