Published in
American Society for Microbiology, Infection and Immunity, 10(68), p. 6082-6086, 2000
DOI: 10.1128/iai.68.10.6082-6086.2000
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Peritoneal Culture Alters Streptococcus pneumoniae Protein Profiles and Virulence Properties
,
Christopher W. Robb,
David A. Watson,
Watson Da,
David W. Niesel
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Abstract
ABSTRACT We have examined the properties of Streptococcus pneumoniae cultured in the murine peritoneal cavity and compared its virulence-associated characteristics to those of cultures grown in vitro. Analysis of mRNA levels for specific virulence factors demonstrated a 2.8-fold increase in ply expression and a 2.2-fold increase in capA3 expression during murine peritoneal culture (MPC). Two-dimensional gels and immunoblots using convalescent-phase patient sera and murine sera revealed distinct differences in protein production in vivo (MPC). MPC-grown pneumococci adhered to A549 epithelial cell lines at levels 10-fold greater than those cultured in vitro.