The aim of this study was to assess the effect of an experimental diet (ED), with high levels of 18:1n-9 and low EPA/ARA ratio, on the fatty acid (FA) profile of ovary and eggs of Seriola dumerili broodstock, in contrast to a non-specific commercial diet (nsCD), taking wild fish lipid composition as a positive reference. Two groups of Seriola broodstock born in captivity were fed with either the ED or the nsCD during two consecutive spawning seasons (21 months). After seven months of feeding, fish fed the ED displayed an ovary FA profile much closer to wild fish. During the second spawning season, only the group fed ED released eggs. Egg FA composition experienced some minor changes throughout the spawning season, being a marginal reduction of EPA in the late season the most striking variation. Overall, the use of the ED showed some positive results, which could favour spontaneous egg releasing of females born in captivity. However the lack of fertilization and the high level of 18:2n-6 in ovary tissue and eggs indicate that further improvements are needed in Seriola dumerili broodstock diet formulation in order to enhance the reproductive performance of this species in captivity.Practical Applications: The use of the ED resulted in an ovary fatty acid profile of cultured females that better resembles that of wild fish. The lower EPA/ARA ratio present in the ED compared to the nsCD tested, could have favoured spontaneous egg releasing of S.dumerili females born in captivity. Thus, using broodstock diets with balanced EPA/ARA ratios (close to wild fish) may have a positive effect on fish broodstock reproductive performance, at least for this species.