Low glomerular (nephron) endowment has been associated with an increased risk of cardiovascular and renal disease in adulthood. Nephron endowment in humans is determined by 36 wk of gestation, while in rats and mice nephrogenesis ends several days after birth. Specific genes and environmental perturbations have been shown to regulate nephron endowment. Until now, design-based method for estimating nephron number in developing kidneys was unavailable. This was due in part to the difficulty associated with unambiguously identifying developing glomeruli in histological sections. Here, we describe a method that uses lectin histochemistry to identify developing glomeruli and the physical disector/fractionator principle to provide unbiased estimates of total glomerular number ( N_glom). We have characterized N_glom throughout development in kidneys from 76 rats and model this development with a 5-parameter logistic equation to predict N_glom from embryonic day 17.25 to adulthood ( r² = 0.98). This approach represents the first design-based method with which to estimate N_glom in the developing kidney.