The concentration of glycine (Gly) was measured in gray and white matter (GM and WM) in the human brain using single-voxel localized 1H-MRS at 7 T. A point-resolved spectroscopy (PRESS) sequence with TE = 150 ms was used for measuring Gly levels in various regions of the frontal and occipital lobes in eleven healthy volunteers and one subject with a glioblastoma. The PRESS spectra were analyzed with LCModel using basis functions generated from density matrix simulations that included the effects of volume localized radio-frequency and gradient pulses. The fraction of gray and white matter within the voxels was obtained from T1-weighted image segmentation. The metabolite concentrations within the voxels, estimated with respect to the GM+WM water concentrations, were fitted to a linear function of fractional GM content. The Gly concentrations in pure GM and WM were estimated to be 1.1 and 0.1 mM, with 95% confidence intervals 1.0 - 1.2 and 0.0 - 0.2, respectively.