The development of a planar all-polymer transistor based on the conducting polymer poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS), compatible with low-cost production techniques such as ink-jet printing, or roll-to roll processing was studied. Eight organic electrochemical transistor (OECTs) were fabricated on a single glass slide to allow for simultaneous measurements of multiple devices. The glass slide is positioned inside a 3D-printed holder with spring-loaded contact pins for connections. All measurements were done using the PEDOT:PSS film as gate electrode and cell media as the electrolyte. The measurement parameters were chosen to avoid exposing the cell monolayers to a voltage drop above 0.5 V, as high voltages have been shown to damage bilayer membranes. The recording of the OECTs was performed using a Keithley 2612 Source Meter and customized Labview software. The study clearly shows that the measured electrical signal is due to tight junctions (TJ)-related barrier tissue formation rather than simple cell coverage; the presence of cells on the active area of the OECT does not modify the transistor response to gate pulse voltage unless the cells present barrier properties.