ABSTRACT Deep neck space infections are defined as infections that spread along the fascial planes and spaces of the head and neck. Even in the era of antibiotics, these infections can and have been potentially life-threatening conditions. The role of single nucleotide polymorphisms (SNPs) of tumor necrosis factor-a (TNF-a) and transforming growth factor-b1 (TGF-b1) genes in deep neck infections has not been studied. Thus, the aim of this study was to investigate the distribution of the TNF-a G-308A and TGF-b1 C-509T polymorphisms in patients suffering from infections of deep neck spaces and to determine the correlation of these polymorphisms with the values of inflammation markers [C-reactive protein (CRP) and white blood cell (WBC) count]. A total of 41 patients with infections of deep neck spaces and 44 healthy controls were screened for TNF-a G-308A and TGF-b1 C-509T polymorphisms using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The distribution of the TNF-a G-308A genotype in patients did not reveal statistically significant correlation compared to con-trols (p = 0.483, c2 = 0.491) as well as the distribution of the TGF-b1 C-509T genotypes (p = 0.644, c2 = 0.725). The distribution of TNF-a -308 and TGF-b1 -509 alleles was not significantly different in patients compared to controls. Moreover, CRP levels and WBC counts were not associated with TNF-a G-308A and TGF-b1 C-509T promoter polymorphisms in patients with deep neck infections. In conclusion, our study suggests that the TNF-a G-308A and TGF-b1 C-509T polymorphisms are not associated with infections of deep neck spaces