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MyJove Corporation, Journal of Visualized Experiments, 65, 2012

DOI: 10.3791/4163

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Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation

Journal article published in 2012 by Emilie Pacary, Matilda A. Haas, Hendrik Wildner, Roberta Azzarelli, Donald M. Bell ORCID, Djoher Nora Abrous, François Guillemot
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

In utero electroporation (IUE) has become a powerful technique to study the development of different regions of the embryonic nervous system 1-5. To date this tool has been widely used to study the regulation of cellular proliferation, differentiation and neuronal migration especially in the developing cerebral cortex 6-8. Here we detail our protocol to electroporate in utero the cerebral cortex and the hippocampus and provide evidence that this approach can be used to study dendrites and spines in these two cerebral regions.