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Public Library of Science, PLoS ONE, 6(3), p. e2514, 2008

DOI: 10.1371/journal.pone.0002514

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Engineering of a Genetically Encodable Fluorescent Voltage Sensor Exploiting Fast Ci-VSP Voltage-Sensing Movements

Journal article published in 2008 by Alicia Lundby ORCID, Hiroki Mutoh, Dimitar Dimitrov, Walther Akemann, Thomas Knöpfel
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement (‘gating’ current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.