International Union of Crystallography, Acta Crystallographica Section F: Structural Biology Communications, 9(72), p. 677-680, 2016
DOI: 10.1107/s2053230x16011638
Full text: Unavailable
Protein surface layers are self-assembling, paracrystalline lattices on the surface of many prokaryotes. Surface-layer proteins have not benefited from widespread structural analysis owing to their resistance to crystallization. Here, the successful expression of a truncated version of RsaA, the surface-layer protein fromCaulobacter crescentus, from aCaulobacterprotein-expression system is reported. The purification, crystallization and initial X-ray diffraction analysis of the truncated RsaA, the largest surface-layer protein studied to date and the first from a Gram-negative bacterium, are also reported.