Temperature exerts strong controls on the incidence and severity of fire. All else equal, warming is expected to increase fire-related carbon emissions, and thereby atmospheric CO 2 . But the magnitude of this feedback is very poorly known. We use a single-box model of the land biosphere to quantify this positive feedback from satellite-based estimates of biomass burning emissions for 2000–2014 CE and from sedimentary charcoal records for the millennium before the industrial period. We derive an estimate of the centennial-scale feedback strength of 6.5 ± 3.4 ppm CO 2 per degree of land temperature increase, based on the satellite data. However, this estimate is poorly constrained, and is largely driven by the well-documented dependence of tropical deforestation and peat fires (primarily anthropogenic) on climate variability patterns linked to the El Niño–Southern Oscillation. Palaeo-data from pre-industrial times provide the opportunity to assess the fire-related climate–carbon-cycle feedback over a longer period, with less pervasive human impacts. Past biomass burning can be quantified based on variations in either the concentration and isotopic composition of methane in ice cores (with assumptions about the isotopic signatures of different methane sources) or the abundances of charcoal preserved in sediments, which reflect landscape-scale changes in burnt biomass. These two data sources are shown here to be coherent with one another. The more numerous data from sedimentary charcoal, expressed as normalized anomalies (fractional deviations from the long-term mean), are then used – together with an estimate of mean biomass burning derived from methane isotope data – to infer a feedback strength of 5.6 ± 3.2 ppm CO 2 per degree of land temperature and (for a climate sensitivity of 2.8 K) a gain of 0.09 ± 0.05. This finding indicates that the positive carbon cycle feedback from increased fire provides a substantial contribution to the overall climate–carbon-cycle feedback on centennial timescales. Although the feedback estimates from palaeo- and satellite-era data are in agreement, this is likely fortuitous because of the pervasive influence of human activities on fire regimes during recent decades.