Abstract Macroalgae are valuable resources for human consumption in many countries. This work reports for the first time a comparative evaluation of the nutritional properties of five edible macroalgae from the genus Cystoseira, namely C. humilis, C. tamariscifolia, C. nodicaulis, C. compressa and C. baccata. For this purpose, their proximate composition was determined in terms of moisture, ash, and total contents of protein, lipids, carbohydrates and mineral profile. Cystoseira tamariscifolia and C. baccata were the species that in general had the higher ash, protein and lipid contents, while the highest levels of moisture and total carbohydrates were detected in C. nodicaulis and C. compressa. Cystoseira species had also high amounts of minerals, especially of potassium, calcium and iron, and a favorable Na/K ratio. The present study shows that Cystoseira has a balanced nutritional composition, suitable for human consumption, and that its intake can contribute to a healthy and well-balanced diet.