Dissemin is shutting down on January 1st, 2025

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F1000Research, F1000Research, (7), p. 148

DOI: 10.12688/f1000research.13598.1

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Ten steps to get started in Genome Assembly and Annotation

Journal article published in 2018 by Victoria Dominguez del Angel ORCID, Erik Hjerde ORCID, Lieven Sterck ORCID, Salvadors Capella-Gutierrez ORCID, Cederic Notredame, Olga Vinnere Pettersson, Joelle Amselem ORCID, Laurent Bouri ORCID, Stéphanie Bocs ORCID, Christophe Klopp ORCID, Jean-François Gibrat ORCID, Anna Vlasova ORCID, Brane L. Leskosek, Lucile Soler, Mahesh Binzer-Panchal ORCID and other authors.
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

As a part of the ELIXIR-EXCELERATE efforts in capacity building, we present here 10 steps to facilitate researchers getting started in genome assembly and genome annotation. The guidelines given are broadly applicable, intended to be stable over time, and cover all aspects from start to finish of a general assembly and annotation project. Intrinsic properties of genomes are discussed, as is the importance of using high quality DNA. Different sequencing technologies and generally applicable workflows for genome assembly are also detailed. We cover structural and functional annotation and encourage readers to also annotate transposable elements, something that is often omitted from annotation workflows. The importance of data management is stressed, and we give advice on where to submit data and how to make your results Findable, Accessible, Interoperable, and Reusable (FAIR).